IHC Polymer-based detection kits - Mouse tissues - Anti-Rat IgG - Alkaline phosphatase (AP)
Antigen detection with primary antibody of the same species as the test tissue yields high background when indirect detection method is used. This severely limits the use of screening human antibody on human tissues.
Our kits are specifically designed to significantly reduce endogenous mouse Ig staining when using rat primary antibodies on mouse tissue. Since the two rat and mouse species are very similar, cross-reactions between the anti-rat IgG antibodies and the mouse immunoglobulins present in the mouse tissue studied
The most commonly used HRP substrates are DAB and AEC. These 2 substrates form brown (DAB) and red (AEC) insoluble precipitates when they are oxidized by HRP. It is for this reason that they are commonly called chromogens. The localization of the target proteins is then carried out by microscopic visualization of the precipitates within the tissue.
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- AP 3
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