Affinity resins for glycobiology - Lectins
Affinity chromatography relies on the specific and reversible binding of a protein to a ligand bound to the matrix, which is then referred to as an affinity resin. The ligand can either bind directly to the protein of interest or to a tag that is covalently attached to the protein (Histidine, GST ...). Affinity chromatography is often the most robust purification procedure and is generally used in the early stages of the purification process. Depending on the downstream application, affinity purification may be the only chromatographic step necessary to achieve adequate purity.
LEctPROFILE gels are affinity gel chromatography where lectins are immobilized on a Sepharose 4B fast flow matrix. Glycoconjugates can be recovered by competition with the specific inhibitory monosaccharide of the lectin. LEctPROFILE gels are used for the purification of glycoconjugates with specific N-glycan residues.
Applications
- Purification of polysaccharides.
- Purification of complex glycoproteins having specific glycans structures.
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