LYZ Chemi-Luminescent ELISA Kit (Chicken)
Cat# OKCD03732
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for LYZ Chemi-Luminescent ELISA Kit (Chicken) (OKCD03732) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Lysozyme (LZM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lysozyme (LZM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lysozyme (LZM) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 13.7-10000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysozyme (LZM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysozyme (LZM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.0137 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | LYZ |
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| Gene Full Name | lysozyme (renal amyloidosis) |
| Alias Symbols | 1,4-beta-N-acetylmuramidase C;4-beta-N-acetylmuramidase C;allergen Gal d IV;dystrophin;egg white lysozyme;lysozyme C;LYZC. |
| NCBI Gene Id | 396218 |
| Protein Name | Lysozyme C |
| Description of Target | Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. |
| Uniprot ID | P00698 |
| Protein Accession # | NP_990612 |
| Nucleotide Accession # | NM_205281 |