LYZ Chemi-Luminescent ELISA Kit (Chicken)
Katalog-Nummer OKCD03732
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for LYZ Chemi-Luminescent ELISA Kit (Chicken) (OKCD03732) |
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Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Lysozyme (LZM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lysozyme (LZM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lysozyme (LZM) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 13.7-10000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysozyme (LZM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysozyme (LZM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.0137 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | LYZ |
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Gene Full Name | lysozyme (renal amyloidosis) |
Alias Symbols | 1,4-beta-N-acetylmuramidase C;4-beta-N-acetylmuramidase C;allergen Gal d IV;dystrophin;egg white lysozyme;lysozyme C;LYZC. |
NCBI Gene Id | 396218 |
Protein Name | Lysozyme C |
Description of Target | Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. |
Uniprot ID | P00698 |
Protein Accession # | NP_990612 |
Nucleotide Accession # | NM_205281 |