| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Bovine, Human, Mouse, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptide (C-MSIRVTQKSYKVST) of human keratin K8 (formerly also designated cytokeratin 8), coupled to KLH |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P05786 (Bovine), P05787 (Human), P11679 (Mouse), Q10758 (Rat) |
| Synonym | Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8 |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:50-1:250 |
| Immunohistochemistry (IHC) - paraffin | 1:50-1:250 (trypsin treatment or microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Specific for keratin K8. Marker for discrimination of simple/non-stratified epithelia from those of stratified origin.
Reactive polypeptide: basic human keratin K8 (Mr 52,000) of all simple type and complex epithelia.
Positive control: human intestinal epithelia.
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