Guinea Pig Polyclonal Anti-Keratin K14

Katalog-Nummer GP-CK14

Size : 100µL

Marke : Progen

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anti-Keratin K14 guinea pig polyclonal, serum

Product: anti-Keratin K14 guinea pig polyclonal, serum
Art.No.: GP-CK14

Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Peptide against human K14 C-VSTHEQVLRTKN (460-472)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P02533 (Human), Q61781 (Mouse)
Synonym Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:5,000-1:10,000

Reactive Polypeptide: acidic keratin K14 (Mr 50,000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis.
Tested cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue).
Tumors specifically detected: the antiserum reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin K14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references).

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