de en

Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-Pan-Nav1 Sodium Channel Antibody

Drucken

Katalog-Nummer 75-405

Size : 100uL

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Antibodies Incorporated

Contact local distributor :

Telefonnummer : +1 850 650 7790

10 Citations

Our Anti-Pan-Nav1 sodium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N419/40. It detects human, mouse, and rat Pan-Nav1 sodium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescence staining of adult rat cortex with N419/40 (red), K89/34 (green) and Hoechst nuclear stain (blue).

$Immunoblot against fractions of adult rat brain membranes (RBM) and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid probed with N419/40 (left) or K58/35 (right) TC supe.$

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Data Sheet

SKU: 75-405

Product Details

Target

Pan-Nav1 sodium channel

Target Description

The family of voltage gated sodium channels comprise a large alpha subunit and auxiliary beta subunits. Nav1 channels are members of the voltage gated sodium ion channel family and form the alpha type, pore-forming subunit of the channel. There are nine known members of this family. These alpha subunits vary in their function and tissue expression.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N419/40

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

SCN2A NAC2 SCN2A1 SCN2A2 SCN1A NAC1 SCN1 SCN9A NENA SCN5A SCN3A KIAA1356 NAC3 SCN8A MED SCN10A SCN4A SCN11A SCN12A SNS2

Molecular Weight

>200 kDa

Antigen

Synthetic peptide amino acids 1501-1518 (TEEQKKYYNAMKKLGSKK, cytoplasmic loop between repeat III and IV) of human Nav1.1 (accession number P35498)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2491098

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

Cross-reacts with multiple Nav1 channels

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Sodium channel protein type 2 subunit alpha (HBSC II) (Sodium channel protein brain II subunit alpha) (Sodium channel protein type II subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.2) Sodium channel protein type 1 subunit alpha (Sodium channel protein brain I subunit alpha) (Sodium channel protein type I subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.1) Sodium channel protein type 9 subunit alpha (Neuroendocrine sodium channel) (hNE-Na) (Peripheral sodium channel 1) (PN1) (Sodium channel protein type IX subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.7) Sodium channel protein type 5 subunit alpha (HH1) (Sodium channel protein cardiac muscle subunit alpha) (Sodium channel protein type V subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.5) Sodium channel protein type 3 subunit alpha (Sodium channel protein brain III subunit alpha) (Sodium channel protein type III subunit alpha) (Voltage-gated sodium channel subtype III) (Voltage-gated sodium channel subunit alpha Nav1.3) Sodium channel protein type 8 subunit alpha (Sodium channel protein type VIII subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.6) Sodium channel protein type 10 subunit alpha (Peripheral nerve sodium channel 3) (PN3) (hPN3) (Sodium channel protein type X subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.8) Sodium channel protein type 4 subunit alpha (SkM1) (Sodium channel protein skeletal muscle subunit alpha) (Sodium channel protein type IV subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.4) Sodium channel protein type 11 subunit alpha (Peripheral nerve sodium channel 5) (PN5) (Sensory neuron sodium channel 2) (Sodium channel protein type XI subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.9)

UniProt Details

UniProt (Human): P35498
UniProt (Immunogen Species): P35498

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34302896	not listed	da Silva, T.F., et al. 2021. Plasmalogens regulate the AKT-ULK1 signaling pathway to control the position of the axon initial segment. Progress in Neurobiology, 102123.

Immunocytochemistry: Rat
PMID	Dilution	Publication
32116557	1:100	Hefting, L.L., et al. 2020. Multiple Domains in the Kv7.3 C-Terminus Can Regulate Localization to the Axon Initial Segment. Frontiers in Cellular Neuroscience, 10.
30914445	1:1000	Solé, L., et al. 2019. The MAP1B Binding Domain of Nav1.6 Is Required for Stable Expression at the Axon Initial Segment. Journal of Neuroscience, 4238-4251.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35354025	not listed	Kaneko, K., et al. 2022. Developmentally regulated impairment of parvalbumin interneuron synaptic transmission in an experimental model of Dravet syndrome. Cell reports, 110580.
33335032	1:100	Yang, S.M., et al. 2020. Neuron class-specific responses govern adaptive myelin remodeling in the neocortex. Science, eabd2109.
31933626	1:300	Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
31201232	1:4 (supe)	Hosoi, N., et al. 2019. Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments. Journal of Neuroscience, 6339-6353.

Western Blot: Mouse
PMID	Dilution	Publication
31933626	1:500	Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
31437534	1:500	Roberts, A.J., et al. 2019. Increased IL-6 expression in astrocytes is associated with emotionality, alterations in central amygdala GABAergic transmission, and excitability during alcohol withdrawal. . Brain, Behavior, and Immunity., 188-202.

Western Blot: Rat
PMID	Dilution	Publication
26644354	not listed	Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities. New Biotechnology, 551-564.