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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-Kv7.1/KCNQ1 K+ Channel Antibody

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Katalog-Nummer 75-081

Size : 100uL

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Antibodies Incorporated

Contact local distributor :

Telefonnummer : +1 850 650 7790

13 Citations

Our Anti-Kv7.1/KCNQ1 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N37A/10. It detects hamster, human, mouse, rabbit, rat, and Xenopus Kv7.1/KCNQ1 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Transfected cell immunofluorescence: COS-1 cells expressing KCNQ1. Red = N37A/10, Blue = Hoechst nuclear stain.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM (left lane) and COS-1 cells transiently transfected with flag-tagged KCNQ1 (middle lane) or Kv2.1 (right lane) plasmids and probed with pure N37A/10 (left panel), rabbit anti-flag (middle panel) or K89/41 TC supe (right panel).

Species Reactivity

Hamster, Human, Mouse, Rabbit, Rat, Xenopus

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Data Sheet

SKU: 75-081

Product Details

Target

Kv7.1/KCNQ1 K+ channel

Target Description

Potassium voltage-gated channel subfamily Q member 1 is encoded by the gene KCNQ1. KCNQ1 is a member of the potassium channel family, KQT (TC 1.A.1.15) subfamily, Kv7.1/KCNQ1 sub-subfamily. KCNQ1 is a voltage-gated potassium channel that is required for the repolarization phase of the cardiac action potential. KCNQ1 can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. KCNQ1 is expressed in the heart, pancreas, kidney, prostate, small intestine, and peripheral blood leukocytes. Diseases associated with KCNQ1 include Long QT Syndrome1, Jervell and Lange-Nielsen Syndrome, atrial fibrillation familial 3, short QT syndrome 2, and atrial fibrillation.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N37A/10

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

KCNQ1 KCNA8 KCNA9 KVLQT1

Molecular Weight

75 kDa

Antigen

Fusion protein amino acids 2-101 of human KCNQ1(accession number P51787) produced recombinantly in E. Coli

Species Reactivity

Hamster, Human, Mouse, Rabbit, Rat, Xenopus

Antibody Registry ID

AB_2131682

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.11

Dilution Ranges

WB: 1:500
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily KQT member 1 (IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1) (KQT-like 1) (Voltage-gated potassium channel subunit Kv7.1)

UniProt Details

UniProt (Human): P51787
UniProt (Immunogen Species): P51787

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Hamster
Western Blot: Hamster | Mouse | Rabbit| Rat | Xenopus

Immunocytochemistry: Mouse
PMID	Dilution	Publication
35085542	1:100	Paventi, G., et al. 2022. Kv7.4 channels regulate potassium permeability in neuronal mitochondria. Biochemical pharmacology, 114931.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
25965962	1:50	Salomonsson, M., et al. 2015. K(V)7.4 channels participate in the control of rodent renal vascular resting tone. Acta physiologica (Oxford, England), 402-414.

Immunohistochemistry: Rat
PMID	Dilution	Publication
25965962	1:50	Salomonsson, M., et al. 2015. K(V)7.4 channels participate in the control of rodent renal vascular resting tone. Acta physiologica (Oxford, England), 402-414.
24109455	0.6μg/ml	Evseev, A., et al. 2013. Functional effects of KCNQ K(+) channels in airway smooth muscle. Frontiers in physiology, 277.

Immunoprecipitation: Hamster
PMID	Dilution	Publication
19907016	not listed	Harmer, S., et al. 2010. Mechanisms of disease pathogenesis in long QT syndrome type 5. American journal of physiology. Cell physiology, C263-273.

Western Blot: Hamster
PMID	Dilution	Publication
19907016	not listed	Harmer, S., et al. 2010. Mechanisms of disease pathogenesis in long QT syndrome type 5. American journal of physiology. Cell physiology, C263-273.

Western Blot: Mouse
PMID	Dilution	Publication
35085542	1:500	Paventi, G., et al. 2022. Kv7.4 channels regulate potassium permeability in neuronal mitochondria. Biochemical pharmacology, 114931.
23504710	1:200	Sachse, C., et al. 2013. BACE1 and presenilin/γ-secretase regulate proteolytic processing of KCNE1 and 2, auxiliary subunits of voltage-gated potassium channels. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2458-2467.
19443733	not listed	Bajwa, P., et al. 2009. Activation of PPARgamma by rosiglitazone attenuates intestinal Cl- secretion. American journal of physiology. Gastrointestinal and liver physiology, G82-89.

Western Blot: Rabbit
PMID	Dilution	Publication
28655649	not listed	Dressler, F., et al. 2017. Interregional electro-mechanical heterogeneity in the rabbit myocardium. Progress in biophysics and molecular biology, 344-355.

Western Blot: Rat
PMID	Dilution	Publication
26718475	1:500	Testai, L., et al. 2016. Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection. Cardiovascular research, 40-50.

Western Blot: Xenopus
PMID	Dilution	Publication
22024150	1:200	Krzystanek, K., et al. 2012. Deubiquitylating enzyme USP2 counteracts Nedd4-2-mediated downregulation of KCNQ1 potassium channels. Heart rhythm, 440-448.