Vicia villosa Lectin (VVA, VVL) (FITC)

Katalog-Nummer V2121-94C-2mg

Size : 2mg

Marke : US Biological

Contact local distributor :


Telefonnummer : +1 850 650 7790


Conjugate
FITC

Grade
Molecular Biology Grade

Applications
FLISA

Shipping Temp
Blue Ice

Storage Temp
-20°C

Hairy Vetch, Fodder Vetch, Winter Vetch
This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially alpha- or beta-linked terminal N-acetylgalactosamine, especially a single alpha N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the “Tn antigen”). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (a-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. Fluorescein labeled Vicia Villosa Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Vicia Villosa Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Applications:
Suitable for use in FLISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Inhibiting/Eluting Sugar:
200mM N-acetylgalactosamine

Excitation:
495-500nm

Emission:
514-521nm

F/P (molar):
3.5

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Source
Vicia villosa (hairy vetch) seeds

Concentration
~1mg/ml

Form
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++. Labeled with Fluorescein isothiocyanate (FITC).


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.