Ulex europaeus (UEA II)

Katalog-Nummer 518886-2mg

Size : 2mg

Marke : US Biological

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Telefonnummer : +1 850 650 7790


Grade
Affinity purified

Shipping Temp
Blue Ice

Storage Temp
-20°C

UEA II
Affinity purified Ulex europaeus agglutinin (UEA II) is isolated from gorse seeds and separated from crude extract. This lectin is a glycoprotein that consists of four 24,000 Da monomer subunits that require Ca2+ for binding to its ligands through carbohydrate recognition domain. It is specific for di-N-acetylchitobiose, an oligomer of GlcNAc. The monosaccharide GlcNAc is not an inhibitor of the lectin activity. UEA II has no mitogenic activity and agglutinates type O erythrocytes with greater interest than type A3 erythrocytes. Addition of EDTA eliminates agglutinating activity. Agglutinating activity can be recovered with the addition of calcium.UEA II is a potent inhibitor of the lectin complement pathway following endothelial oxidative stress. The molecular mechanism of UEA II-induced inhibition of the lectin complement pathway appears to be through competition for a common binding site on the endothelial cell and not at the level of mannose-binding lectin (MBL) inhibition. See other UEA II conjugates.

Source:
Gorse

Blood Specificity:
Non-specific

Sugar Specificity:
Oligomers of beta(1,4) - linked N-Acetylglucosamine

Inhibitory Carbohydrate:
GlcNAc beta(1,4) GlcNAc

Divalent Ions:
Ca++, Mn++, Zn++

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Gorse

Purity
Affinity purified

Form
Supplied as a lyophilized powder.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.