Triticum vulgaris Lectin (WGA) - Cy3

    Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. 

   Similar to insulin, WGA enhances the rate of glucose oxidation in isolated fat cells and is used for isolation and fractionation of insulin receptors. A recent time-of-addition study made it evident that WGA inhibits infection with SARS-CoV-2 by inhibiting replication when the lectin is incubated with the virus and added to cells during infection. The data from the same study indicated the mode of action is an interaction between WGA and the viral envelope, causing neutralization of the virus. It is possible this occurs via the highly glycosylated spike proteins. WGA has been used in oral squamous cell carcinoma staining techniques to test the degree of cell cohesion. It has been shown to inhibit melanoma cell proliferation in a dose-dependent manner. Cytotoxic activity on cancer cell lines is characteristic of WGA. The effects of its binding have been studied in a plethora of cancers including but not limited to hepatoma, choriocarcinoma, osteosarcoma, and some pancreatic cancers. 

      Cy3 can be used to visualize the binding pattern of Triticum vulgaris Lectin (WGA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical

Specifications:

• Source: Triticum vulgaris (Wheat germ)
• Activity: 10 µg/ml will agglutinate human erythrocytes nonspecifically.
• Carbohydrate Specificity: N-Acetylglucosamine
• Inhibitory Carbohydrate: Chitin hydrolysate or 500 mM N-Acetylglucosamine
• Mitogenic Activity: Yes
• Divalent Ions Required: None
• Conjugation: Cy3
• Excitation:  Maximum at 550 nm
• Emission: Maximum at 570 nm
• Fluorescence: Orange

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

Application

Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Mitogenic Stimulation

References

Auth, J., Fröba, M., Große, M., Rauch, P., Ruetalo, N., Schindler, M., Morokutti-Kurz, M., Graf, P., Dolischka, A., Prieschl-Grassauer, E., Setz, C., & Schubert, U. (2021). Lectin from Triticum vulgaris (WGA) Inhibits Infection with SARS-CoV-2 and Its Variants of Concern Alpha and Beta. International journal of molecular sciences, 22(19), 10205. https://doi.org/10.3390/ijms221910205

Johnson, R. J., Simpson, S., Van Epps, D. E., & Chenoweth, D. E. (1992). Wheat germ agglutinin inhibits the C5a receptor interaction: implications for receptor microheterogeneity and ligand binding site. Journal of leukocyte biology, 52(1), “10. https://doi.org/10.1002/jlb.52.1.3

Loréa, P., Goldschmidt, D., Darro, F., Salmon, I., Bovin, N., Gabius, H. J., Kiss, R., & Danguy, A. (1997). In vitro characterization of lectin-induced alterations on the proliferative activity of three human melanoma cell lines. Melanoma research, 7(5), “363. https://doi.org/10.1097/00008390-199710000-00001

Schwarz, R. E., Wojciechowicz, D. C., Picon, A. I., Schwarz, M. A., & Paty, P. B. (1999). Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells. British journal of cancer, 80(11), “1762. https://doi.org/10.1038/sj.bjc.6690593

Silveyra, E., Bologna-Molina, R., Gónzalez-Gónzalez, R., & Arocena, M. (2021). The Tissue Architecture of Oral Squamous Cell Carcinoma Visualized by Staining Patterns of Wheat Germ Agglutinin and Structural Proteins Using Confocal Microscopy. Cells, 10(9), 2466. https://doi.org/10.3390/cells10092466

Wang, H., Ng, T. B., Ooi, V. E., & Liu, W. K. (2000). Effects of lectins with different carbohydrate-binding specificities on hepatoma, choriocarcinoma, melanoma and osteosarcoma cell lines. The international journal of biochemistry & cell biology, 32(3), “372. https://doi.org/10.1016/s1357-2725(99)00130-2