TCEP (hydrochloride) [51805-45-9]
Katalog-Nummer HY-W011500-1ml
Size : 10mM/1mL
Marke : MedChemExpress
Contact local distributor :
Telefonnummer : +1 850 650 7790
TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
TCEP hydrochloride Chemische Struktur
CAS. Nr. : 51805-45-9
* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.
This product is a controlled substance and not for sale in your territory.
- •Mol Cell. 2023 Nov 20:S1097-2765(23)00914-0. [Abstract]
- •Acta Biomater. 2023 Dec 8:S1742-7061(23)00710-9. [Abstract]
- •Cell Biosci. 2022 Dec 21;12(1):206. [Abstract]
- •Int J Mol Sci. 2023 Dec 18;24(24):17631. [Abstract]
Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung
| TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry. | |
| In Vitro | TCEP hydrochloride has been introduced which oers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
|---|---|
|
-
[1]. Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14(1):7-16.
[2]. Duchardt F, et al. A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. J Biol Chem. 2009 Dec 25;284(52):36099-108.
[3]. Sequeira MA, et al. Modulating amyloid fibrillation in a minimalist model peptide by intermolecular disulfide chemical reduction. Phys Chem Chem Phys. 2019 Jun 5;21(22):11916-11923.
[4]. Wu R, et al. Effects of Small Molecules on DNA Adsorption by Gold Nanoparticles and a Case Study of Tris(2-carboxyethyl)phosphine (TCEP). Langmuir. 2019 Oct 15;35(41):13461-13468.
[5]. Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994;220(1):5-10.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|
| H2O / DMSO | 1 mM | 3.4886 mL | 17.4429 mL | 34.8857 mL | 87.2144 mL |
| 5 mM | 0.6977 mL | 3.4886 mL | 6.9771 mL | 17.4429 mL | |
| 10 mM | 0.3489 mL | 1.7443 mL | 3.4886 mL | 8.7214 mL | |
| 15 mM | 0.2326 mL | 1.1629 mL | 2.3257 mL | 5.8143 mL | |
| 20 mM | 0.1744 mL | 0.8721 mL | 1.7443 mL | 4.3607 mL | |
| 25 mM | 0.1395 mL | 0.6977 mL | 1.3954 mL | 3.4886 mL | |
| 30 mM | 0.1163 mL | 0.5814 mL | 1.1629 mL | 2.9071 mL | |
| 40 mM | 0.0872 mL | 0.4361 mL | 0.8721 mL | 2.1804 mL | |
| 50 mM | 0.0698 mL | 0.3489 mL | 0.6977 mL | 1.7443 mL | |
| 60 mM | 0.0581 mL | 0.2907 mL | 0.5814 mL | 1.4536 mL | |
| 80 mM | 0.0436 mL | 0.2180 mL | 0.4361 mL | 1.0902 mL | |
| 100 mM | 0.0349 mL | 0.1744 mL | 0.3489 mL | 0.8721 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Others
- Others
- Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
TCEP51805-45-9Tris(2-carboxyethyl)phosphineOthersInhibitorinhibitorinhibit
Ihre kürzlich angesehenen Produkte:
Online-Anfrage
Your information is safe with us. * Required Fields.
Massenanfrage
Inquiry Information
- Produktname:
- TCEP hydrochloride
- Art. -Nr.:
- HY-W011500
- Menge:
- MCE Japan Authorized Agent: