SepFast DUO 5000 Q
Katalog-Nummer 510104-25ML
Size : 25ml
Marke : Biotoolomics
Introduction
SepFast DUO™ IEX is a range of unique shelled ion-exchange resins. Unlike ViralPolish® and MabPolish DUO, the core chemistries of the SepFast DUO™ media are classic ion-exchangers (Q or S).
SepFast DUO™ can be used for purifying viral materials in flow-through mode, impurities smaller than a virus will be adsorbed to the charged groups inside the bead whilst intact virus passes through the column. It is a very gentle method with little loss of product. Because the interior core has a standard ion-exchange ligand impurities can be eluted and the beads easily regeneration making this approach useful for large scale multi-use columns.
SepFast DUO 150 Q and SepFast DUO 150 S are particularly developed for purifying antibodies in flow-through mode. Host cell proteins (HCP) smaller than Ab molecules can be selectively removed.
SepFast DUO™ is available in four different outer shell pore sizes and two different inner core chemistries (see Table below)
Shelled IEX Resin | Application Guide | Key Features |
SepFastTM DUO 5000Q SepFastTM DUO 5000S | Good for lentivirus, adeno virus etc. AAV may be purified if very high molecular-weight impurity (e.g. truncated AAV or empty capsid) need be removed. | The inert out shell has molecular cut-off of 5000 KDa, 700 KDa, 400 KDa and 150 KDa, respectively, to block viruses or antibodies. Strong ion-exchange ligand is immobilised inside to capture impurities. Q: strong anion-exchanger S: strong cation-exchanger |
SepFastTM DUO 700Q SepFastTM DUO 700S | Good for AAVs, other viruses and VLPs of similar sizes, plasmids | |
SepFastTM DUO 400Q SepFastTM DUO400S | Good for certain vaccine antigens, large proteins etc | |
SepFastTM DUO 150Q SepFastTM DUO 150S | Good for monoclonal antibodies |
Characteristics of SepFastTM DUO IEX Range
Particle size | 50 - 150 µm |
Ligand | Q: quaternary S: sulfopropyl |
Ligand density | 70-150 µmol / ml resin |
Protein binding capacity | Depends on the type of proteins and binding conditions |
Operational pressure | Up to 3 bar |
Flow velocity* | Up to 500 cm/hr |
Chemical stability | Stable in most common aqueous buffers |
pH stability | 2-14 (<2 h) 3-12 (long term) |
Storage | 20% ethanol at 4oC |
*Linear flow rate = volumetric flow rate (c/column cross-sectional area (cm2)
*Other column size available on request.