EasyPure® Plant Genomic DNA Kit (with RNase A）
Size : 200rxns
Marke : TransGen Biotech
EasyPure® Plant Genomic DNA Kit provides a simple and convenient way to isolate high quality plant genomic DNA from plant tissues (up to 100 mg). Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent. Proteins, polysaccharides, and cell debris are eliminated by precipitation. DNA is bound to silica-based column.
• DNA yield up to 15 μg.
• Complete removal of pigment, polysaccharides and other impurity.
• DNA range of 20-50 kb.
• Column based purification, no organic extraction or ethanol precipitation.
PCR, restriction enzyme digestion and Southern blotting
at room temperature (15-25°C) in a dry place for one year.
at room temperature
|Component||EE111-01 (50 rxns)||EE111-02 (200 rxns)||EE111-11 (50 rxns)||EE111-12 (200 rxns)|
|Resuspension Buffer1 (RB1)||15 ml||60 ml||15 ml||60 ml|
|Precipitation Buffer1 (PB1)||6 ml||25 ml||6 ml||25 ml|
|Binding Buffer1 (BB1)||8 ml||32 ml||8 ml||32 ml|
|Clean Buffer 1 (CB1)||30 ml||110 ml||30 ml||110 ml|
|Wash Buffer 1 (WB1)||12 ml||2×22 ml||12 ml||2×22 ml|
|Elution Buffer (EB)||25 ml||80 ml||25 ml||80 ml|
|RNase A (10 mg/ml)||800 μl||4×800 μl||0||0|
|Genomic Spin Column with Collection Tubes||50 each||200 each||50 each||200 each|
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