EasyPure® Plant Genomic DNA Kit (with RNase A）
Katalog-Nummer EE111-02
Size : 200rxns
Marke : TransGen Biotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
ManualEasyPure® Plant Genomic DNA Kit provides a simple and convenient way to isolate high quality plant genomic DNA from plant tissues (up to 100 mg). Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent. Proteins, polysaccharides, and cell debris are eliminated by precipitation. DNA is bound to silica-based column.
• DNA yield up to 15 μg.
• Complete removal of pigment, polysaccharides and other impurity.
• DNA range of 20-50 kb.
• Column based purification, no organic extraction or ethanol precipitation.
Applications
PCR, restriction enzyme digestion and Southern blotting
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature
| Component | EE111-01 (50 rxns) | EE111-02 (200 rxns) | EE111-11 (50 rxns) | EE111-12 (200 rxns) |
| Resuspension Buffer1 (RB1) | 15 ml | 60 ml | 15 ml | 60 ml |
| Precipitation Buffer1 (PB1) | 6 ml | 25 ml | 6 ml | 25 ml |
| Binding Buffer1 (BB1) | 8 ml | 32 ml | 8 ml | 32 ml |
| Clean Buffer 1 (CB1) | 30 ml | 110 ml | 30 ml | 110 ml |
| Wash Buffer 1 (WB1) | 12 ml | 2×22 ml | 12 ml | 2×22 ml |
| Elution Buffer (EB) | 25 ml | 80 ml | 25 ml | 80 ml |
| RNase A (10 mg/ml) | 800 μl | 4×800 μl | 0 | 0 |
| Genomic Spin Column with Collection Tubes | 50 each | 200 each | 50 each | 200 each |
Zhang Y , Li Z X , Yu X D , et al. Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation[J]. New Phytologist, 2015, 206(3):1101-1115.
Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.
Liu P , Qi M , Wang Y , et al. Arabidopsis RAN1 Mediates Seed Development through Its Parental Ratio by Affecting the Onset of Endosperm Cellularization[J]. Molecular Plant, 2014, 7(8):1316-1328.
Chen K , Han H , Luo Z , et al. A practicable detection system for genetically modified rice by SERS-barcoded nanosensors[J]. Biosensors and Bioelectronics, 2012, 34(1):118-124.
Bai N , Wang S , Abuduaini R , et al. Rhamnolipid-aided biodegradation of carbendazim by, Rhodococcus, sp. D-1: Characteristics, products, and phytotoxicity[J]. Science of The Total Environment, 2017, 590-591:343-351.