Visualize Bands in 2 Minutes. All nUView precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Run Times from 30 Minutes. Reduces the time to run the gels.
Increased Resolution. nUView Tris-Glycine gels are formulated to run at a constant 250 volts rather than 150 volts for the Laemmli gel (when using the Tris-Glycine/SDS running buffer). The higher voltage reduces the run time to 30 minutes compared to the 90 minutes with the Laemmli gel while maintaining a high resolution separation.
Longer Shelf Life. With a 24-month shelf life (4°C) from date of manufacture or 6 months at room temperature (25°C), NuSep gels provide reproducible results time after time.
Broader Migration Range. nUView Tris-Glycine gels offer greater resolution across a wider mass range (205 kDa to 2.5kDa). Migration patterns are available in a broad range of formulations and well configurations.
Choice. You can choose from 3 different cassette sizes:
- NB: Fits Mini Protean™ tanks.
- NN: Fits XCell SureLock™ Mini-Cell tanks.
- NG: Compatible with all other 10cm tanks.
Easy to Use. NuSep precast gels are easy to use and contain a tear open pouch and tapeless cassette for fast set up plus trouble-free cassette loading and opening (requiring no key or knife to open).
All the cassettes have large sample well capacities (up to 50 μL) with numbered lanes and solid well dividers for easy well identification – eliminating cross sample contamination and gel damage.
Running Buffers. We will recommend to use Tris-Glycine SDS running buffer (BG-143)
Accessories. NuSep offers a broad range of buffers, stains and drying solutions.
Satisfies Standard Protocols. All standard western blotting protocols and all standard in-gel digestion protocols for Mass Spectrometry analysis can be used with NuSep nUView gels.
Let nUView Tris-Glycine gels improve your workflow … save time and get better results faster.
What is the NuSep Advantage?
As a small company, we put extra time and effort into making our products work for your research. We are willing to take the extra time and effort to provide our customers with the best service and ensure a quality experience. We strive to provide researchers with the right tools to help them advance forward. nUView Tris-Glycine Precast Gels contains NuSep’s patented solid gel well technology.
Why solid gel wells?
Solid gel wells with plastic dividers differs from other precast gels because with the plastic dividers you no longer need to worry about gel wells changing shape and getting distorted. The solid wells diminish the chance of distorted gel wells. This also allows us to remove the combs prior to shipping the gel. This means that researchers no longer need to spend time removing the comb or worrying about distorting gel wells and taking the time to fix it. We are here to make your research easier and more efficient.
*NG series relabeled from Dec. 2018
Cat# NG21 converts to Cat# NG10 (10-well)
Cat# NG11 converts to Cat# NG12 (12-well)
Cat# NG31 converts to Cat# NG15 (15-well)
Convert to nUView Tris-Glycine Precast Gels
Convert from Bio-Rad Mini-PROTEAN TGX Gels to NuSep nUView Precast Gels
Convert from Invitrogen Bolt Bis-Tris to NuSep nUView Precast Gels
Convert from Invitrogen NuPAGE Bis-Tris to NuSep nUView Precast Gels
Convert from Invitrogen Novex Tris-Glycine to NuSep nUView Precast Gels
Select Your nUView Tris-Glycine Precast Gels
*NG series relabeled from Dec. 2018
Cat# NG21 converts to Cat# NG10 (10-well)
Cat# NG11 converts to Cat# NG12 (12-well)
Cat# NG31 converts to Cat# NG15 (15-well)
NuSep Tris-Glycine gels can be developed in a range of Tris buffers with different counter ions, each producing a subtle variation to the migration pattern.
NuSep provides a range of cassettes to suit your needs – no need to replace your current tank!
NB cassettes (10cm wide X 8.5cm high) will fit in all Bio-Rad™ Mini-Protean™ tanks (including tetra and dodeca cells).
NN cassettes (10cm wide X 10cm high) will fit in Mini gel Tank or Invitrogen™ X-Cell Sure-Lock™ Mini-Cell Tank.
NG cassettes (10cm wide X 8.0cm high) fit in all other 10cm tanks:
• VWR Tanks
• GradiGel Mini 4 -Cell
• EC 4- Cell
• Hoefer Tall Mighty Small (SE 280)
• Hoefer Mighty Small II (SE 260/SE 250)
• Hoefer Mini VE
• Daiichi Mini 2-Gel & 6-Gel
• Owl Road Runner, Penguin
• Invitrogen™ Novex® XCell I and II and SureLock™
• Bio-Rad® Mini-PROTEAN® II & 3
• Owl Single Sided Vertical System
• Shelton IBI protein system
Buffer Formulations
Running Buffers (10x)
| Running Buffer Recipe | Tris-HEPES-SDS | Tris-Glycine-SDS | Tris-Tricine-SDS |
| Tris | 121 g | 29 g | 61 g |
| HEPES | 238 g | – | – |
| Gylcine | – | 144 g | – |
| Tricine | – | – | 9 g |
| SDS | 10 g | ||
| Deionized Water | To 1000 mL | ||
TruSep Sample Buffer (2x, 10mL, no reducing agent)
| Component | Amount |
| Tris | 0.15 g |
| SDS | 0.4 g |
| Bromophenol Blue | 0.003 g |
| Glycerol | 4 mL |
| 5 M Hydrochloric Acid | 0.25 mL |
Western Blotting Protocol
| Condition | Wet-Blotting | Semi-Dry Blotting |
| Voltage | 40 V | 20 V |
| Time | 120 min | 30 min |
| Recommended Buffer | NuSep transfer buffer* | |
| Membrane | Nitrocellulose or PVDF | |
*Standard Tris-Glycine can be used. Protocol may need to be optimized.
Compatible Buffers
• Tris-Glycine (available from NuSep)
Compatible Stains
All proteins stains are compatible with nUView gels, including:
• Coomassie blue
• Silver and copper stains
• Fluorescent stains
FREQUENTLY ASKED QUESTIONS
How do I read catalog numbers?
The first two letters represents the gel size:
NB – NuSep Bio-rad (fits Bio-rad’s tank)
NG – NuSep General (fits generic tanks – 10cm)
NN – NuSep Novex (fits Thermo Fisher’s tanks)
The two numbers following the first two letters represents the number of wells, and the last three numbers represents the %Acrylamide.
For example: NN10-008 means you have a gel that fits Novex’s tanks, it has 10 wells, and has 8% Acrylamide.
For example: NB17-816 means you have a gel that fits Bio-rad’s tanks, it has 17 wells, and has 8-16% Acrylamide.
*NG series relabeled from Dec. 2018
Cat# NG21 converts to Cat# NG10 (10-well)
Cat# NG11 converts to Cat# NG12 (12-well)
Cat# NG31 converts to Cat# NG15 (15-well)
Do you sell gradient gels?
Yes.
• 8-16% for mid-range separations
• 4-20% for wide-range separations
Do I have to buy a new tank?
No. NuSep gels are compatible with most popular mini-gel tanks.
Do they have a stacker gel?
All the gels have a 4% stacking gel.
Do they contain SDS?
No, the gels do not contain SDS (SDS is incorporated in the running buffer solution). The gels can be used for native runs.
Can I use reducing agent?
NuSep gels and sample buffer do not contain reducing agents. They can be added to a sample prior to loading. Beta-mercaptoethanol and DTT are both suitable for use with our gels. See Running formulations.
What is the gel thickness?
1mm
Can the gels be dried?
Yes. NuSep gels must be air dried. Use of vacuum drying results in cracking. NuSep gels can only be dried crack-free with NuSep Gel Drying Solution. All other drying solutions will cause cracking. A recommended gel drying protocol can be found on the NuSep website.
TROUBLE SHOOTING
Problem: Distorted protein bands.
Cause: Air bubbles in the sample wells, or between gel and cassette, or at the bottom of the cassette.
Solution: Use a transfer pipette to displace the air bubbles from the sample wells.
Problem: Streaking.
Cause: Poorly soluble or weakly charged particles (such as carbohydrates) in sample.
Solution:
1 Centrifuge sample.
2 Change pH of buffer.
3 Heat sample in the presence of SDS.
Problem: Bands difficult to distinguish.
Cause: Incorrect gel selection, sample overloading, insufficient cooling buffer.
Solution:
1 Reduce sample size.
2 Select a gel which separates in the desired molecular weight range.
3 For proteins of similar molecular weight a 2-D separation may be required.
4 Increase buffer in outer tank.
Problem: Sample spreading across the gel.
Cause: Too much salt in the sample.
Solution: Reduce salt by dialysis or ultra-filtration.
Problem: Sample contains appreciable carbohydrate.
Solution: Enzymatically remove the carbohydrate.
Problem: Sample contains lipoproteins.
Solution: Use a gel with large pore size at top. Try addition of a non-ionic detergent.
Problem: Protein denaturation and band inversion.
Cause: Excessive heating.
Solution: Always start with chilled buffer (<15°C).
Problem: Diffuse proteins zones in gel after staining.
Cause: SDS still present in gel.
Solution: Fix in 10% TCA prior to staining, otherwise use 30% methanol in the destaining solution.
Protocols:
nUView Precast Gels UV Visualization
nUView Precast Gels Instructions for Use
nUView Precast Gels Western Blotting Protocol
MSDS:
nUView Tris-Glycine Precast Gels MSDS
Tris-Glycine SDS Running Buffer (10x) MSDS
Convert to nUView Precast gels
Convert from Bio-Rad Mini-Protean to NuSep nUView Precast Gels
Convert from Invitrogen Bolt Bis-Tris to NuSep nUView Precast Gels
Convert from Invitrogen NuPAGE Bis-Tris to NuSep nUView Precast Gels
Convert from Invitrogen Novex Tris-Glycine to NuSep nUView Precast Gels