SARS-CoV-2 ORF3a Gene Tagged ORF (codon optimized)

CAT#: VC102558

  • TrueORF®

Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391. Note: ORF is codon optimized


Plasmid Map

Product Images

Specifications

Product Data
Tag Myc-DDK
Symbol ORF3a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>VC102558 representing YP_009724391, codon optimized.
Due to the complexity of YP_009724391, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACCTCTTTATGCGCATTTTTACCATTGGCACCGTAACTCTCAAACAAGGTGAAATAAAGGACGCC
ACCCCATCAGATTTTGTCCGAGCTACAGCAACCATCCCTATACAGGCATCCCTGCCTTTCGGCTGGCTC
ATTGTTGGGGTCGCCCTTCTGGCCGTATTTCAATCCGCATCAAAAATTATCACTCTGAAGAAACGCTGG
CAGCTGGCTCTGTCTAAAGGCGTGCACTTCGTCTGTAATCTCCTTCTCCTGTTTGTTACCGTCTACTCT
CATCTTTTGCTCGTGGCTGCCGGCCTGGAAGCCCCATTTCTGTATCTCTATGCCCTCGTCTATTTTCTC
CAATCAATTAATTTCGTTAGGATTATCATGCGCCTCTGGTTGTGCTGGAAATGCCGCTCCAAAAACCCC
CTGCTCTATGACGCTAACTACTTCCTTTGCTGGCACACCAACTGCTATGATTACTGCATCCCCTATAAC
TCTGTTACAAGTAGCATTGTGATCACATCTGGCGACGGCACAACTTCTCCAATCTCCGAGCACGATTAT
CAAATTGGTGGCTATACTGAAAAATGGGAAAGTGGCGTAAAAGACTGCGTGGTGCTGCACTCCTATTTC
ACATCCGATTATTACCAACTCTATTCAACCCAACTGTCAACTGATACAGGCGTCGAACACGTGACGTTC
TTTATTTATAATAAGATTGTCGATGAACCTGAAGAACACGTACAGATCCACACAATCGACGGATCATCT
GGCGTCGTCAACCCCGTAATGGAGCCCATCTACGACGAACCAACAACTACTACCTCTGTACCTCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC102558 representing YP_009724391
Blue=ORF Red=Cloning site Green=Tag(s)

MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRW
QLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNP
LLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYF
TSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NC_045512
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq YP_009724391
RefSeq ORF 825 bp
MW 31.1 kDa

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