Rat TIMP-1 ELISA Kit

CAT#: EA100402

Rat TIMP-1 ELISA Kit


Product Images

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat TIMP-1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat TIMP-1
Sensitivity <3pg/ml
Reactivities Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat TIMP-1 antibody 1
  • Lyophilized recombinant rat TIMP-1 standard 10ng/tubex2
  • Biotinylated anti- rat TIMP-1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.
Gene Symbol Timp1
Gene ID 116510

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