Protein G SepFast Large Bead
Katalog-Nummer 230601-1L
Size : 1litre
Marke : Biotoolomics
Contact local distributor :
Telefonnummer : +1 850 650 7790
Introduction
Protein G SepFast is a family of affinity resins with protein G immobilized to highly cross-linked agarose-based beads of various sizes.
- Protein G SepFast HighRes: 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate)
- Protein G SepFast: 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
- Protein G SepFast Large Bead: 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure)
Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.
The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Protein G SepFast is developed and supported for production scale chromatography. BioToolomics offers both loose medium and pre-packed ready-to-use disposable columns.
Characteristics of Protein G SepFast Range
| Matrix | Highly cross-linked agarose bead |
| Particle size | 20 - 50 µm Protein G SepFast HighRes50 - 150 µm Protein G SepFast 150 - 350 µm Protein G SepFast Large Bead |
| Max. operating pressure | 0.3 MPa (3 bar, 42 psi) |
| Recommended flow rate* | 50 to 300 cm/h |
| Binding capacity** | ≥ 15 mg human IgG/mL resin |
| Chemical compatibility | The commonly used reagents for antibody purifications |
| Chemical stability | Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11. |
| Physical stability | Negligible volume variation due to changes in pH or ionic strength. |
| pH stability | 3 to 9 (long term); 2 to 10 (short term) |
| Sanitization | Sanitize the column with 70% ethanol |
| Storage & temperature | 20% ethanol at 2oC – 8oC |
*Other column size available on request.