Protein A Green Separopore® 4B Microbeads viewIP™
Katalog-Nummer 20181108-1
Size : 2mL
Marke : BioWorld
Description
Applications:
The green-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the green beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Green-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
References
References:
Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646. J Bacteriol. (1997) 179: 3482-7.
Purification and characterization of bovine interstitial collagenase and tissue inhibitor of metalloproteinases. Arch Biochem Biophys. (1992) 293: 370-6.
Properties
Storage Temperature
2-8°C
ECCN #
EAR99
Related Products
20181041,20181105,20181118
Hazmat Ship
Non-hazardous
Matrix
Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle Size Range
52 - 165 μm
Matrix Activation
Cyanogen bromide
Binding Capacity
~ 20 mg human IgG/ml drained gel
Suspension/Column/Cartridge
Supplied as 50% suspension in PBS containing 50% glycerol
pH Stability
3-9 (for use), 2-10 (for cleaning)
Molecular Weight Exclusion limit
approximately 2 x 107