Protein A Green Separopore® 4B Microbeads viewIP™

Katalog-Nummer 20181108-1

Size : 2mL

Marke : BioWorld

Contact local distributor :


Telefonnummer : +1 850 650 7790

Description

Applications:

The green-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the green beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Green-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646. J Bacteriol. (1997) 179: 3482-7.
Purification and characterization of bovine interstitial collagenase and tissue inhibitor of metalloproteinases. Arch Biochem Biophys. (1992) 293: 370-6.

Properties

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Matrix

Separopore® 4B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

~ 20 mg human IgG/ml drained gel

Suspension/Column/Cartridge

Supplied as 50% suspension in PBS containing 50% glycerol

pH Stability

3-9 (for use), 2-10 (for cleaning)

Molecular Weight Exclusion limit

approximately 2 x 107