Anti-Occludin Antibody
Katalog-Nummer CQA2850-50ul
Size : 50ul
Marke : Cohesion Biosciences
Contact local distributor :
Telefonnummer : +1 850 650 7790
- Western blot analysis of Occludin expression in HEK293T (A), mouse brain (B), rat liver (C) whole cell lysates.
- Immunohistochemical analysis of Occludin staining in mouse pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
- Immunofluorescent analysis of Occludin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
| Product Name | Anti-Occludin Antibody |
| Cat No: | CQA2850 |
| Source: | Rabbit |
| Reactivity: | H, M, R |
| Applications: | WB, IH, IF/IC |
| *Application Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
| *Species Reactivity Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
Description: Rabbit polyclonal antibody to Occludin Immunogen: Recombinant full length protein of human Occludin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: OCLN Alternative Names: OccludinEntrez Gene (Human): Entrez Gene (Mouse) : Entrez Gene (Rat) : SwissProt (Human): SwissProt (Mouse) : SwissProt (Rat) : Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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