Vhl Mouse Gene Knockout Kit (CRISPR)
CAT#: KN318888LP
Vhl - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Vhl |
| Locus ID | 22346 |
| Components | KN318888G1, Vhl gRNA vector 1 in pCas-Guide CRISPR vector KN318888G2, Vhl gRNA vector 2 in pCas-Guide CRISPR vector KN318888LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_009507 |
| UniProt ID | P40338 |
| Synonyms | Vhlh |
| Summary | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN318888 | Vhl - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN318888BN | Vhl - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN318888RB | Vhl - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN518888 | Vhl - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA204595 | Vhl CRISPRa kit - CRISPR gene activation of mouse von Hippel-Lindau tumor suppressor | 1 657,00 EUR |