Vash1 (NM_177354) Mouse Tagged ORF Clone

CAT#: MR222520

  • TrueORF®

Vash1 (Myc-DDK-tagged) - Mouse vasohibin 1 (Vash1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Vash1
Synonyms AI834978; D930046M13Rik; G630009D10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222520 representing NM_177354
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGGGGAAAGAAGGTGGTCCCAAGTGGCAGCAGCAGTGCCTCCCCGAACGCAGCCGCCACCACCA
CTGCTGCTGCTGCTGCTGCCGCTGCTGCCCCCCACTCTGGCACTAAACGTTTGGAGACCACCGAAGGGGC
CTCAGCACAGAGAGATGAGGAACCCGAAGAGGAAGGGGAAGAGGACCTACGAGATGGAGGTGTCCCTTTT
TTCATCAACCGAGGTGGACTGCCAGTGGATGAGGCCACCTGGGAAAGGATGTGGAAGCATGTGGCCAAGA
TCCACCCAGATGGAGAGAAGGTGGCCCTGCGCATCCGTGGGGCCACCGACCTGCCCAAGATTCCCATACC
AAGTGTGCCTACTTTCCAGCCGACGACGCCCGTCCCTGAGCGCCTGGAAGCCGTGCAGCGCTACATCAGG
GAGCTGCAGTACAATCACACAGGGACACAATTCTTTGAAATTAAGAAGAGCAGACCTCTGACGGGGCTGA
TGGACCTGGCCAAGGAAATGACCAAAGAGGCTCTGCCAATCAAATGCCTGGAAGCTGTGATCCTGGGAAT
TTACCTCACCAACAGTATGCCCACCCTGGAACGCTTTCCCATCAGCTTCAAGACCTATTTCTCAGGGAAC
TACTTCCGCCACATTGTGCTGGGGGTGAACTTCGGGGGCCGCTATGGCGCCCTGGGTATGAGCCGAAGGG
AGGACCTGATGTACAAACCGCCCGCCTTCCGCACGCTCAGTGAGCTCGTGCTGGACTACGAAGCGGCCTA
CGGCCGCTGCTGGCACGTGCTGAAGAAGGTGAAGCTGGGTCAGTGTGTGTCCCACGACCCGCACAGTGTG
GAACAGATTGAATGGAAGCACTCGGTGCTGGATGTGGAGAGGCTGGGGCGGGAAGACTTCCGCAAGGAGC
TGGAGCGTCATGCCCGAGACATGCGGCTCAAGATTGGCAAGGGGACAGGCCCTCCTTCTCCCACCAAGGA
CCGGAAGAAGGATGTTTCCTCCCCACAGAGGGCCCAGTCCAGCCCCCATCGCAGGAACAGCCGCAGTGAG
AGACGGCCTTCAGGGGAGAAGAAGCCCGCAGAGCCCAAAGCCATGCCAGACCTCAGTGGGTACCAGATCC
GGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR222520 representing NM_177354
Red=Cloning site Green=Tags(s)

MPGGKKVVPSGSSSASPNAAATTTAAAAAAAAAPHSGTKRLETTEGASAQRDEEPEEEGEEDLRDGGVPF
FINRGGLPVDEATWERMWKHVAKIHPDGEKVALRIRGATDLPKIPIPSVPTFQPTTPVPERLEAVQRYIR
ELQYNHTGTQFFEIKKSRPLTGLMDLAKEMTKEALPIKCLEAVILGIYLTNSMPTLERFPISFKTYFSGN
YFRHIVLGVNFGGRYGALGMSRREDLMYKPPAFRTLSELVLDYEAAYGRCWHVLKKVKLGQCVSHDPHSV
EQIEWKHSVLDVERLGREDFRKELERHARDMRLKIGKGTGPPSPTKDRKKDVSSPQRAQSSPHRRNSRSE
RRPSGEKKPAEPKAMPDLSGYQIRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_177354
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_177354.4, NP_796328.2
RefSeq Size 6189 bp
RefSeq ORF 1128 bp
Locus ID 238328
UniProt ID Q8C1W1
Cytogenetics 12 D2
MW 42.3 kDa
Gene Summary Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146868). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC213050 Vash1 (untagged) - Mouse vasohibin 1 (Vash1), (10ug)
    • 10 ug

503,00 EUR

MG222520 Vash1 (tGFP-tagged) - Mouse vasohibin 1 (Vash1), (10ug)
    • 10 ug

657,00 EUR

MR222520L3 Lenti ORF clone of Vash1 (Myc-DDK-tagged) - Mouse vasohibin 1 (Vash1)
    • 10 ug

757,00 EUR

MR222520L4 Lenti ORF clone of Vash1 (mGFP-tagged) - Mouse vasohibin 1 (Vash1)
    • 10 ug

757,00 EUR