TYR ELISA Kit (Bovine)

Katalog-Nummer OKCD02965

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for TYR ELISA kit (Bovine)
Product Info
Predicted Species ReactivityBovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)95-105%83-96%80-102%88-96%
EDTA Plasma (n=5)93-101%89-96%89-101%98-105%
Heparin Plasma (n=5)93-101%96-104%97-104%82-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosinase (TYR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosinase (TYR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosinase (TYR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tyrosinase (TYR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.81-500ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)82-9986
EDTA Plasma (n=5)78-9084
Heparin Plasma(n=5)90-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosinase (TYR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosinase (TYR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TYR Microplate96 Wells (12 x 8 Well strips)
TYR Lyophilized Standard2
100X Biotinylated TYR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.1 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tyrosinase (TYR). No significant cross-reactivity or interference between Tyrosinase (TYR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolTYR
Alias SymbolsMonophenol monooxygenase, Tyrosinase
NCBI Gene Id280951
Protein NameTyrosinase
Description of TargetThis is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Uniprot IDQ8MIU0
Protein Accession #NP_851344.1
Nucleotide Accession #NM_181001.3
Protein Size (# AA)530