Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit at 1/1,0000 dilution Predicted band size: 59,42 kDa Observed band size: 58 kDa
Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit at 1/1,0000 dilution Predicted band size: 59,42 kDa Observed band size: 58 kDa
Recombinant Human Zinc finger protein RFP protein (214-513AA)
Purification
Affinity Purified
Preservative
0.02% sodium azide
Formulation
PBS with, 50% glycerol, pH7.3.
Reference
[1]"Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination."Hao Y.H., Doyle J.M., Ramanathan S., Gomez T.S., Jia D., Xu M., Chen Z.J., Billadeau D.D., Rosen M.K., Potts P.R.Cell 152:1051-1064(2013). [2]"Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2beta." Cai X., Srivastava S., Sun Y., Li Z., Wu H., Zuvela-Jelaska L., Li J., Salamon R.S., Backer J.M., Skolnik E.Y.Proc. Natl. Acad. Sci. U.S.A. 108:20072-20077(2011). [3]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010).
ret finger protein;RFP;RFP transforming protein;RING finger protein 76;RING-type E3 ubiquitin transferase TRIM27;RNF76;tripartite motif protein TRIM27;tripartite motif-containing protein 27;zinc finger protein RFP.
NCBI Gene Id
5987
Protein Name
Zinc finger protein RFP
Description of Target
E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.
What is the species homology for "TRIM27 Antibody (OACA07077)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
How long will it take to receive "TRIM27 Antibody (OACA07077)"?
This item is available "Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks".
What buffer format is "TRIM27 Antibody (OACA07077)" provided in?
This item is provided in "Liquid". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "TRIM27 Antibody (OACA07077)"?
This target may also be called "ret finger protein;RFP;RFP transforming protein;RING finger protein 76;RING-type E3 ubiquitin transferase TRIM27;RNF76;tripartite motif protein TRIM27;tripartite motif-containing protein 27;zinc finger protein RFP." in publications.
What is the shipping cost for "TRIM27 Antibody (OACA07077)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "TRIM27 Antibody (OACA07077)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "TRIM27 Antibody (OACA07077)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "TRIM27 Antibody (OACA07077)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "TRIM27"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "TRIM27"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "TRIM27"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "TRIM27"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "TRIM27"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "TRIM27"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
Positive Control: Human Muscle" data-size="300x300" class="product-image-wrapper cover">
TRIM27 Antibody - middle region (ARP34701_P050)
Catalog #: ARP34701_P050
Species Tested: Human
Application: WB
Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
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