Total MMP-1 ELISA Kit (Human)
Katalog-Nummer OKAG00170
Size : 1plate:12x8-WellMicrostrips
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||||||
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | 4.5 hours | ||||||||||||||||||||||
ELISA Kit Principle | The Aviva Human MMP-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hMMP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human MMP-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||||||
ELISA Kit Range | 157-10000 pg/ml | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||
Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||||||
Sensitivity | 157 pg/mL | ||||||||||||||||||||||
Specificity | The Human MMP-1 ELISA is capable of recognizing both recombinant and naturally produced Human MMP-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human: MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, MMP-13, MMP-16, TIMP-1, TIMP-2, TIMP-3, TIMP-4 | ||||||||||||||||||||||
Assay Info | Quantitative Colorimentric Sandwich ELISA |
Gene Symbol | MMP1 |
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Gene Full Name | matrix metallopeptidase 1 |
Alias Symbols | CLG;CLGN;fibroblast collagenase;interstitial collagenase;matrix metallopeptidase 1 (interstitial collagenase);matrix metalloprotease 1;Matrix metalloproteinase-1. |
NCBI Gene Id | 4312 |
Protein Name | P03956 |
Description of Target | Interstitial collagenase |
Uniprot ID | https://www.uniprot.org/uniprot/P03956 |
Protein Accession # | NP_002412.1 |
Nucleotide Accession # | NM_002421.3 |