Tbc1d7 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN317258BN
Tbc1d7 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro RFP-BSD
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
| Symbol | Tbc1d7 |
| Locus ID | 67046 |
| Components | KN317258G1, Tbc1d7 gRNA vector 1 in pCas-Guide CRISPR vector KN317258G2, Tbc1d7 gRNA vector 2 in pCas-Guide CRISPR vector KN317258BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001252639, NM_001252640, NM_025935 |
| UniProt ID | Q9D0K0 |
| Synonyms | 2610009C09Rik |
| Summary | Component of the TSC-TBC complex, that contains TBC1D7 in addition to the TSC1-TSC2 complex and consists of the functional complex possessing GTPase-activating protein (GAP) activity toward RHEB in response to alterations in specific cellular growth conditions. The small GTPase RHEB is a direct activator of the protein kinase activity of mTORC1 and the TSC-TBC complex acts as a negative regulator of mTORC1 signaling cascade by acting as a GAP for RHEB. Participates in the proper sensing of growth factors and glucose, but not amino acids, by mTORC1. It is unclear whether TBC1D7 acts as a GTPase-activating protein and additional studies are required to answer this question.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN317258 | Tbc1d7 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN317258LP | Tbc1d7 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN317258RB | Tbc1d7 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN517258 | Tbc1d7 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA207106 | Tbc1d7 CRISPRa kit - CRISPR gene activation of mouse TBC1 domain family, member 7 | 1 657,00 EUR |