Stx17 (NM_026343) Mouse Tagged ORF Clone

CAT#: MR204194

  • TrueORF®

Stx17 (Myc-DDK-tagged) - Mouse syntaxin 17 (Stx17)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Stx17
Synonyms 4833418L03Rik; 6330411F21Rik; 9030425C21Rik; AW048351
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAAGATGAGGAAAAGGTGAAGTTACGCAGGCTTGAGCCAGCGATACAGAAATTCACTAAGATCG
TGATCCCAACAGACCTGGAGAGGCTAAGAAAACACCAGATAAACATCGAGAAGTATCAGCGGTGCAGAAT
TTGGGACAAGTTGCACGAAGAGCACATCAACGCAGGGCGCACGGTTCAGCAACTGCGCTCCAATATCCGA
GAAATGGAGAAGCTTTGCTTGAAAGTGCACAAGGATGATCTGGTCCTTTTGAAGCGAATGATAGATCCTG
TCAAGGAAGCAGCAGCAACAGCTACGGCAGAGTTCCTCCAGCTCCACTTGGAATCTGTTGAAGAACTCAA
GAAACAAGTCAACGATGAAGAATTACTTCAGCCTTCTCTGACCAGATCCACAACTGTTGATGGAGTGCTC
CACACTGGGGAGGCTGAAGCTGCTTCTCAGAGTCTGACTCAGATATATGCCTTGCCTGAAATTCCTCAAG
ACCAAAATGCTGCTGAGTCCTGGGAAACCTTAGAAGCGGACTTAATTGAGCTGAGCCATCTGGTCACTGA
CATGTCCCTCCTAGTGAGTTCTCAGCAGGAGAAGATTGACAGCATTGCAGACCATGTCAACAGTGCCGCT
GTGAATGTTGAAGAGGGAACCAAAAACTTGCAGAAGGCTGCAAAATACAAGCTGGCAGCTCTGCCTGTGG
CAGGTGCACTCATCGGAGGAGTGGTAGGGGGTCCCATCGGCCTCCTCGCAGGCTTCAAAGTGGCAGGAAT
TGCAGCTGCACTTGGTGGTGGGGTGTTAGGCTTCACAGGTGGAAAATTGATACAAAGAAGGAAACAGAAA
ATGATGGAGAAGCTCACTTCCAGCTGTCCAGATCTCCCTAGCCAAAGTGACAAAAAACGCAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR204194 protein sequence
Red=Cloning site Green=Tags(s)

MSEDEEKVKLRRLEPAIQKFTKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHINAGRTVQQLRSNIR
EMEKLCLKVHKDDLVLLKRMIDPVKEAAATATAEFLQLHLESVEELKKQVNDEELLQPSLTRSTTVDGVL
HTGEAEAASQSLTQIYALPEIPQDQNAAESWETLEADLIELSHLVTDMSLLVSSQQEKIDSIADHVNSAA
VNVEEGTKNLQKAAKYKLAALPVAGALIGGVVGGPIGLLAGFKVAGIAAALGGGVLGFTGGKLIQRRKQK
MMEKLTSSCPDLPSQSDKKRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026343
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_026343.2, NP_080619.2
RefSeq Size 5777 bp
RefSeq ORF 906 bp
Locus ID 67727
UniProt ID Q9D0I4
Cytogenetics 4 B1
MW 33.2 kDa
Gene Summary SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC210614 Stx17 (untagged) - Mouse syntaxin 17 (Stx17), (10ug)
    • 10 ug

330,00 EUR

MG204194 Stx17 (tGFP-tagged) - Mouse syntaxin 17 (Stx17)
    • 10 ug

500,00 EUR

MR204194L3 Lenti ORF clone of Stx17 (Myc-DDK-tagged) - Mouse syntaxin 17 (Stx17)
    • 10 ug

600,00 EUR

MR204194L4 Lenti ORF clone of Stx17 (mGFP-tagged) - Mouse syntaxin 17 (Stx17)
    • 10 ug

600,00 EUR