SLC27A5 ELISA Kit (Bovine)
Katalog-Nummer OKEH01944
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bovine | ||||||||||||||||||||||
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Application | ELISA | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SLC27A5 ELISA Kit (Bovine) (OKEH01944) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine SLC27A5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC27A5 and incubated. The SLC27A5 found in the sample or standards competes with the biotinylated SLC27A5 for limited binding sites on the immobilized anti-bovine SLC27A5 antibody. Excess unbound biotinylated SLC27A5 and sample or standard SLC27A5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC27A5 captured in the well and inversely proportional to the amount of SLC27A5 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312 - 20 ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.3% (n = 20) Mean Inter-assay CV%: < 8.3% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | < 0.23 ng/mL | ||||||||||||||||||||||
Specificity | This assay recognizes recombinant and natural Bovine BACS. No significant cross-reactivity or interference was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Immunoassay |
Gene Symbol | SLC27A5 |
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