SELE ELISA Kit (Bovine)
Katalog-Nummer OKEH07233
Size : 96T
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
SELE ELISA Kit (Bovine) (OKEH07233)
| Datasheets/Manuals | Printable datasheet for SELE ELISA Kit (Bovine) (OKEH07233) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology SELE ELISA Kit (Bovine) (OKEH07233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SELE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SELE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SELE captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.6% (n = 20) Mean Inter-assay CV%: <=8.4% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. | ||||||||||||||||||||||
| :: | Subcellular Location: Cell membrane Single-pass type I membrane protein | ||||||||||||||||||||||
| :: | Unigene: Bt.197 SMR: P98107 String: 9913.ENSBTAP00000055695 | ||||||||||||||||||||||
| :: | KEGG: bta:281484 Pfam: PF00008 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.15ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine E-selectin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | SELE |
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| Gene Full Name | selectin E |
| Alias Symbols | CD62 antigen-like family member E, E-selectin, ELAM-1, endothelial adhesion molecule 1, endothelial leukocyte adhesion molecule 1, LECAM2, leukocyte-endothelial cell adhesion molecule 2. |
| NCBI Gene Id | 281484 |
| Protein Name | E-selectin |
| Description of Target | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. |
| Uniprot ID | P98107 |
| Protein Accession # | NP_776606.1 |
| Nucleotide Accession # | NM_174181.2 |