Ponceau S [6226-79-5]
Katalog-Nummer HY-12489-500mg
Size : 500mg
Marke : MedChemExpress
Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red.
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Ponceau S Chemische Struktur
CAS. Nr. : 6226-79-5
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Beschreibung |
Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3]. |
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In Vitro |
Ponceau S 的浓度 ( 0.001-2 (w/v); 5-10 分钟) 、酸浓度和酸类型 (乙酸、三氯乙酸/磺基水杨酸),不影响蛋白质检测的灵敏度[1]。 MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molekulargewicht |
760.57 |
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Formel |
C22H12N4Na4O13S4 |
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CAS. Nr. |
6226-79-5 |
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Appearance |
Solid |
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Color |
Brown to black |
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SMILES |
O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O |
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Versand | Room temperature in continental US; may vary elsewhere. |
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Speicherung |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Lösungsmittel & Löslichkeit |
In Vitro:
DMSO : 100 mg/mL (131.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 35.71 mg/mL (46.95 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Reinheit & Dokumentation |
Purity: ≥98.0% |
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Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 1.3148 mL | 6.5740 mL | 13.1480 mL | 32.8701 mL |
5 mM | 0.2630 mL | 1.3148 mL | 2.6296 mL | 6.5740 mL | |
10 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2870 mL | |
15 mM | 0.0877 mL | 0.4383 mL | 0.8765 mL | 2.1913 mL | |
20 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6435 mL | |
25 mM | 0.0526 mL | 0.2630 mL | 0.5259 mL | 1.3148 mL | |
30 mM | 0.0438 mL | 0.2191 mL | 0.4383 mL | 1.0957 mL | |
40 mM | 0.0329 mL | 0.1644 mL | 0.3287 mL | 0.8218 mL | |
DMSO | 50 mM | 0.0263 mL | 0.1315 mL | 0.2630 mL | 0.6574 mL |
60 mM | 0.0219 mL | 0.1096 mL | 0.2191 mL | 0.5478 mL | |
80 mM | 0.0164 mL | 0.0822 mL | 0.1644 mL | 0.4109 mL | |
100 mM | 0.0131 mL | 0.0657 mL | 0.1315 mL | 0.3287 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ponceau S Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Ponceau S6226-79-5Acid Red 112Acid Red112Acid Red-112Fluorescent DyestainWesternblottingacidicantibody-antigenproteinsredInhibitorinhibitorinhibit