PickMutant™ Error Prone PCR Kit

Katalog-Nummer MT040

Size : 30rxn

Marke : Canvax Biotech

Contact local distributor :


Telefonnummer : +1 850 650 7790

PickMutant™ Error Prone PCR Kit

For a Reliable, Robust & highly Efficient Random Mutagenesis by Error-Prone PCR

PickMutant™ Error-Prone PCR Kit is based on the protocol of Cadwell and Joyce (1992). epPCR takes  advantage of the inherently low fidelity of Taq DNA Polymerase (with errors biased toward AT to GC changes), which may be further decreased by the addition of Mn2+, increasing the Mg2+ concentration, and using unequal dNTP concentrations. The rate of Mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0 %.

30 rxn
SKU: MT040 Category: Mutagenesis

Detailed information:

  • Highly Efficient.
  • Reliable.
  • Robust.

– 50 μL Taq Polymerase (5 U/μL)
– 200 μL 10x ep-PCR Buffer
– 80 μL ep-dNTP Mix (unbalanced dNTP ratio)
– 200 μL MnCl2 Solution (25mM)
– 2 x 1.5 mL Water  (PCR Grade)

Datasheet
MSDS
  • Generation of a library of mutants.
  • In vitro Molecular evolution.

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  • Tested using the insert control DNA provided.
  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
  • Khlusevich, Y., Matveev, A., Emelyanova, L., Goncharova, E., Golosova, N., Pereverzev, I., & Tikunova, N. (2022). New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization. Viruses14(6), 1224.
  • Wong, T. S., & Tee, K. L. (2020). Gene Mutagenesis. In A Practical Guide to Protein Engineering (pp. 121-148). Springer, Cham.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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