Application
| FC |
|---|---|
| Isotype | Mouse IgG2a, kappa |
| Concentration | 0.2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Host | Mouse |
| Gene ID | 19264 |
|---|---|
| Alternative Name(s) | SJL, Ly5.1 |
| Format | PerCP-Cy5.5 |
| Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
| Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
| Storage Conditions | 2-8°C protected from light |
Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry)
Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)
Wakim LM, Woodward-Davis A, and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)
Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)
Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)