Nlrp12 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN311043RB
Nlrp12 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Nlrp12 |
| Locus ID | 378425 |
| Components | KN311043G1, Nlrp12 gRNA vector 1 in pCas-Guide CRISPR vector KN311043G2, Nlrp12 gRNA vector 2 in pCas-Guide CRISPR vector KN311043RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001033431 |
| UniProt ID | E9Q5R7 |
| Synonyms | Nalp12; PYPAF7 |
| Summary | Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN311043 | Nlrp12 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN311043BN | Nlrp12 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN311043LP | Nlrp12 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN511043 | Nlrp12 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA216994 | Nlrp12 CRISPRa kit - CRISPR gene activation of mouse NLR family, pyrin domain containing 12 | 1 657,00 EUR |