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> N(6)-Carboxymethyllysine ELISA Kit

N(6)-Carboxymethyllysine ELISA Kit

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Katalog-Nummer OKEH02605

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology N(6)-Carboxymethyllysine ELISA Kit (OKEH02605) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-N(6)-Carboxymethyllysine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated N(6)-Carboxymethyllysine and incubated. The N(6)-Carboxymethyllysine found in the sample or standards competes with the biotinylated N(6)-Carboxymethyllysine for limited binding sites on the immobilized anti-N(6)-Carboxymethyllysine antibody. Excess unbound biotinylated N(6)-Carboxymethyllysine and sample or standard N(6)-Carboxymethyllysine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated N(6)-Carboxymethyllysine captured in the well and inversely proportional to the amount of N(6)-Carboxymethyllysine which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.0% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-N(6)-Carboxymethyllysine Microplate96 Wells (12 x 8 Well strips)
N(6)-Carboxymethyllysine Lyophilized Standard2 x 50 ng
Sample Diluent1 x 20 mL
100X N(6)-Carboxymethyllysine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: CML
Target Name: N(6)-Carboxymethyllysine
::Pubchem: 15691192
::Chemical formula: C8H16N2O4
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.25 ng/mL
SpecificityNatural and recombinant General N(6)-Carboxymethyllysine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsCML, N(6)-Carboxymethyllysine, CML
Protein NameN(6)-Carboxymethyllysine
Description of TargetN(6)-Carboxymethyllysine (CML), also known as N(epsilon)-(carboxymethyl)lysine, is an advanced glycation endproduct (AGE). CML has been the most used marker for AGEs in food analysis.