Product Description  

Mut Express MultiS Fast Mutagenesis Kit V2 is a kit based on ClonExpress rapid cloning technology, which can simultaneously introduce site-directed mutagenesis to 3 - 5 discontinuous sites on the target plasmid. The kit integrates the Phanta Max Super-Fidelity DNA Polymerase amplification system and the ClonExpress rapid cloning system. With superior fidelity, Phanta Max significantly reduces the possibility of introducing novel mutations during amplification. Its excellent long fragment amplification ability is widely applicable to the amplification of any plasmid less than 20 kb. The ClonExpress rapid cloning system replaces the conventional annealing-cyclization reactions with the more efficient homologous recombination reaction. Site-directed mutagenesis using the Mut Express MultiS Fast Mutagenesis Kit V2 allows for more flexible primer design and extremely low template input, thereby facilitating complete degradation of the initially methylated template. The kit is equipped with the highly optimized recombination reaction buffer and the recombinase Exnase MultiS, which specially optimized for multi-base site-directed mutagenesis. If the amplification product is highly specific, its DpnI-digested product can be used directly in the recombination reaction without DNA purification. This product is the preferred kit for DNA multiple point mutation.

 Features  

• Low template usage: Robust and reliable exponential amplification method

• High-fidelity enzyme: Phanta Max Super-Fidelity DNA Polymerase(~128X Taq) minimizes unwanted secondary mutations

• Simple operation: Amplified product can be directly used in the recombination reaction after being digested with DpnI

• Widely applicable: suitable for amplification of any site in the plasmid within 20 kb

• Software support: Provide online primer design software ‘‘CE Design’’

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.

▲Please avoid repeated freezing and thawing.