Mouse APC (Activated Protein C) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Proc
Gene ID:	19123
Uniprot ID:	PROC_MOUSE
Specificity:	This kit recognizes Mouse APC in samples. No significant cross-reactivity or interference between Mouse APC and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function	Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
Protein Name	Vitamin K-Dependent Protein C Anticoagulant Protein C Autoprothrombin Iia Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide
Database Links	Reactome: R-MMU-140837 Reactome: -MMU-140875 Reactome: -MMU-159740 Reactome: -MMU-159763 Reactome: -MMU-159782 Reactome: -MMU-202733 Reactome: -MMU-381426 Reactome: -MMU-8957275
Cellular Localisation	Secreted Golgi Apparatus Endoplasmic Reticulum
Alternative ELISA Names	Vitamin K-Dependent Protein C ELISA kit Anticoagulant Protein C ELISA kit Autoprothrombin Iia ELISA kit Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide ELISA kit Proc ELISA kit
Specificity	This kit recognizes Mouse APC in samples. No significant cross-reactivity or interference between Mouse APC and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-102	97
EDTA plasma (n=8)	91-105	97
Cell culture media (n=8)	88-100	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	260.01	563.69	1988.50	274.76	561.11	1860.49
Standard deviation	16.95	32.02	97.44	16.68	32.21	74.98
CV (%)	6.52	5.68	4.90	6.07	5.74	4.03

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance