LALBA ELISA Kit (Bovine)
Katalog-Nummer OKEH03892
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology LALBA ELISA Kit (Bovine) (OKEH03892) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LALBA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LALBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LALBA captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay Precision - ≤ 5.1%, n = 20 Mean Inter-Assay Precision - ≤ 7.5%, n = 20 | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 1.56 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Alpha-lactalbumin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | LALBA |
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| Gene Full Name | lactalbumin alpha |
| Alias Symbols | a-LACTA;alfaLA;alpha lactabumin;alpha lactalbumin;alpha-lactalbumin;alpha-lactalbumin A;alpha-lactalbumin B;alpha-lactalbumin protein variant D;lactose synthase B protein. |
| NCBI Gene Id | 281894 |
| Protein Name | P00711 |
| Description of Target | Alpha-lactalbumin |
| Uniprot ID | https://www.uniprot.org/uniprot/P00711 |
| Protein Accession # | NP_776803.1 |
| Nucleotide Accession # | NM_174378.2 |
| Protein Size (# AA) | 142 |
| Molecular Weight | 16 kDa |