IL1RL1 ELISA Kit (Rat)
Katalog-Nummer OKCA02562
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Rat | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Il1rl1 ELISA Kit (Rat) (OKCA02562) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il1rl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il1rl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Il1rl1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6 - 1,000 pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Culture Supernates, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 3.9 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | IL1RL1 |
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| Alias Symbols | St2, FIT1, Fit-1 |
| NCBI Gene Id | 25556 |
| Protein Name | Interleukin-1 receptor-like 1 |
| Description of Target | Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. |
| Uniprot ID | Q62611 |
| Protein Accession # | NP_001121161 |
| Nucleotide Accession # | NM_001127689 |