Heparan Sulfate (10E4 epitope) (Biotin)

Katalog-Nummer H1890-01-50ul

Size : 50ul

Marke : US Biological

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Telefonnummer : +1 850 650 7790


H1890-01 Heparan Sulfate (10E4 epitope) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgM,k
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) ß-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-ß-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-ß-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.||Applications:|Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.||Recommended Dilutions: |Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgM,k|Clone No: 8.S.087|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS. Labeled with Biotin.|Purity: Purified by tangential ultrafiltration.|Immunogen: Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.|Specificity: Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.
Form
Supplied as a liquid in PBS. Labeled with Biotin.
Purity
Purified by tangential ultrafiltration.
Specificity
Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).
References
US Biological application references: 1. Geelen, J. et al., (2008) Nephrology Dialysis Transplantation 23:3091-3095. 2. Bacsa, S. et al., (2010) doi:10.1099/vir.0.027052-0. 3. Clément, A. et al, (2008) PLoS Genet 4: e1000136. doi:10.1371/journal.pgen.1000136. General References: 1. David, G., et al., J. Cell Biol. 119: 961-975 (1992). 2. Bai, X.M., et al., J. Histochem. Cytochem. 42: 1043-1054 (1994). 3. Lories, V., et al., J. Biol. Chem. 264: 7009-7016 (1989). 4. Fuxe, K., et al., Brain Res. 636: 131-138 (1994). 5. Nackaerts, K., et al., Int. J. Cancer (Pred. Oncol.) 74: 335-345 (1997). 6. van Kuppevelt, T., et al., J. Biol. Chem. 273: 12,960-12,966 (1998). 7. Yokoyama, H., et al., Kidney Int. 56: 650-658 (1999). 8. Oshiro, M., et al., Histochem. Cell Biol. 115: 373-380 (2001). 9. van den Born, J., et al., J. Biol. Chem. 280: 20,516-20,523 (2005).

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