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> GH Chemi-Luminescent ELISA Kit (Chicken)

GH Chemi-Luminescent ELISA Kit (Chicken)

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Katalog-Nummer OKCD03529

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for GH Chemi-Luminescent ELISA Kit (Chicken) (OKCD03529)
Product Info
Predicted Species ReactivityChicken|Gallus gallus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-95%88-101%91-104%92-102%
EDTA Plasma (n=5)99-105%96-103%92-101%80-102%
Heparin Plasma (n=5)95-104%82-96%96-103%82-92%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Hormone (GH) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9992
EDTA Plasma (n=5)82-9489
Heparin Plasma (n=5)79-9994
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Gh Microplate96 Wells (12 x 8 Well strips)
Gh Lyophilized Standard2
100X Gh Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGh
Alias SymbolsGH1, cGH
NCBI Gene Id378781
Protein NameGrowth hormone
Description of TargetGrowth hormone plays an important role in growth control.
Uniprot IDP08998
Protein Accession #NP_989690
Nucleotide Accession #NM_204359