Equine IL-1 beta ELISA
Katalog-Nummer DIY0699E-004
Size : 1Set
Marke : Kingfisher Biotech
Equine IL-1β ELISA Data
Equine IL-1β ELISA Kit Components
Component | Usage | Quantity | Catalog # |
Anti-Equine IL-1β | Capture Antibody | 100 µg X 2 | KP1013E-100 |
Biotinylated Anti-Equine IL-1β | Detection Antibody | 50 µg | KPB1014E-050 |
Equine IL-1β (IL-1F2) Recombinant Protein | Standard | 5 µg | RP0060E-005 |
Equine IL-1β ELISA Specifications
The Equine IL-1β Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Equine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-1β Background
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta
Feeding Grass Hay Before Concentrate Mitigates the Effect of Grain-Based Concentrates on Postprandial Plasma Interleukin-1β.
Suagee-Bedore JK, Linden DR, Bennett-Wimbush K, Splan RK.
J Equine Vet Sci. 2020 Mar;86:102899. doi: 10.1016/j.jevs.2019.102899. Epub 2019 Dec 24.
Applications: Measurement of equine IL-1 beta in plasma by ELISA
Increasing platelet concentrations in leukocyte-reduced platelet-rich plasma decrease collagen gene synthesis in tendons.
Boswell SG, Schnabel LV, Mohammed HO, Sundman EA, Minas T, Fortier LA.
Am J Sports Med. 2014 Jan;42(1):42-9. doi: 10.1177/0363546513507566. Epub 2013 Oct 17.
Applications: Measurement of equine IL-1 beta in platelet-rich plasma by ELISA