DTNB [69-78-3]
Katalog-Nummer HY-15915-5g
Size : 5g
Marke : MedChemExpress
DTNB (Ellman’s Reagent) is a chemical used to quantify the number or concentration of thiol groups.
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DTNB Chemische Struktur
CAS. Nr. : 69-78-3
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
Beschreibung |
DTNB (Ellman’s Reagent) is a chemical used to quantify the number or concentration of thiol groups[1]. |
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In Vitro |
DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molekulargewicht |
396.35 |
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Formel |
C14H8N2O8S2 |
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CAS. Nr. |
69-78-3 |
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Appearance |
Solid |
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Color |
Off-white to yellow |
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SMILES |
O=C(O)C1=C([N+]([O-])=O)C=CC(SSC2=CC(C(O)=O)=C([N+]([O-])=O)C=C2)=C1 |
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Versand | Room temperature in continental US; may vary elsewhere. |
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Speicherung |
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Lösungsmittel & Löslichkeit |
In Vitro:
DMSO : 100 mg/mL (252.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Reinheit & Dokumentation | |||||||||||||
Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.5230 mL | 12.6151 mL | 25.2302 mL | 63.0756 mL |
5 mM | 0.5046 mL | 2.5230 mL | 5.0460 mL | 12.6151 mL | |
10 mM | 0.2523 mL | 1.2615 mL | 2.5230 mL | 6.3076 mL | |
15 mM | 0.1682 mL | 0.8410 mL | 1.6820 mL | 4.2050 mL | |
20 mM | 0.1262 mL | 0.6308 mL | 1.2615 mL | 3.1538 mL | |
25 mM | 0.1009 mL | 0.5046 mL | 1.0092 mL | 2.5230 mL | |
30 mM | 0.0841 mL | 0.4205 mL | 0.8410 mL | 2.1025 mL | |
40 mM | 0.0631 mL | 0.3154 mL | 0.6308 mL | 1.5769 mL | |
50 mM | 0.0505 mL | 0.2523 mL | 0.5046 mL | 1.2615 mL | |
60 mM | 0.0421 mL | 0.2103 mL | 0.4205 mL | 1.0513 mL | |
80 mM | 0.0315 mL | 0.1577 mL | 0.3154 mL | 0.7884 mL | |
100 mM | 0.0252 mL | 0.1262 mL | 0.2523 mL | 0.6308 mL |
DTNB Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
DTNB69-78-3Ellman’s ReagentBiochemical Assay ReagentsInhibitorinhibitorinhibit