CF®488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL

Katalog-Nummer 20246-1

Size : 50uL

Marke : Biotium

Contact local distributor :


Telefonnummer : +1 850 650 7790

Goat Anti-Mouse IgG1 (γ1)

Goat anti-mouse IgG1 isotype-specific secondary antibody labeled with our superior CF® dyes and biotin.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a goat anti-mouse IgG1 isotype-specific secondary antibody labeled with our bright and photostable CF® Dyes and biotin. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG1. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG2a, IgG2b, IgG3), and human, bovine and rabbit serum proteins.

  • Cross-adsorbed for specific staining with minimal background
  • Available in 12 bright and photostable CF® Dyes
  • Biotin conjugate also available
  • Suitable for western, immunofluorescence, and immunohistology

Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG1

Conjugation Ex/Em Size Catalog No.
CF®350 347/448 nm 50 uL (100 ug) 20245-1
0.25 mL (500 ug) 20245
CF®405S 404/431 nm 50 uL (100 ug) 20380-50uL
0.25 mL (500 ug) 20380-250uL
CF®488A 490/515 nm 50 uL (100 ug) 20246-1
0.25 mL (500 ug) 20246
CF®543 541/560 nm 50 uL (100 ug) 20325-1
0.25 mL (500 ug) 20325
CF®555 555/565 nm 50 uL (100 ug) 20247-1
0.25 mL (500 ug) 20247
CF®568 562/583 nm 50 uL (100 ug) 20248-1
0.25 mL (500 ug) 20248
CF®594 593/614 nm 50 uL (100 ug) 20249-1
0.25 mL (500 ug) 20249
CF®633 630/650 nm 50 uL (100 ug) 20250-1
0.25 mL (500 ug) 20250
CF®640R 642/662 nm 50 uL (100 ug) 20251-1
0.25 mL (500 ug) 20251
CF®647 650/665 nm 50 uL (100 ug) 20252-1
0.25 mL (500 ug) 20252
CF®680 681/698 nm 50 uL (100 ug) 20253-1
0.25 mL (500 ug) 20253
CF®770 770/797 nm 50 uL (100 ug) 20254-1
0.25 mL (500 ug) 20254
Biotin N/A 50 uL (100 ug) 20471-50uL
0.25 mL (500 ug) 20471-250uL

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
92171
 500UG