CCL11 Chemi-Luminescent ELISA Kit (Bovine)
Katalog-Nummer OKCD03415
Size : 96Wells
Marke : Aviva Systems Biology
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for CCL11 Chemi-Luminescent ELISA Kit (Bovine) (OKCD03415) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Eosinophil Chemotactic Factor (ECF) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 5.49-4,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 62.5 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CCL11 |
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| Gene Full Name | chemokine (C-C motif) ligand 11 |
| Alias Symbols | C-C motif chemokine 11;eotaxin;small-inducible cytokine A11. |
| NCBI Gene Id | 404072 |
| Protein Name | Eotaxin |
| Description of Target | Attracts eosinophils in vitro but is not responsible for eosinophilia in the ovary. |
| Uniprot ID | Q9TTS6 |
| Protein Accession # | NP_991342 |
| Nucleotide Accession # | NM_205773 |