- More Files
- Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRCA1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S1423 of human BRCA1.
Sequence
H-G-Sp-Q-P
Host
Rabbit
Theoretical MW (kDa)
220
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells treated with UV (20 min) using BRCA1 (phospho S1423) polyclonal antibody (Cat # PAB25832).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (phospho S1423) polyclonal antibody (Cat # PAB25832). - Gene Info — BRCA1
Entrez GeneID
672Protein Accession#
P38398Gene Name
BRCA1
Gene Alias
BRCAI, BRCC1, IRIS, PSCP, RNF53
Gene Description
breast cancer 1, early onset
Omim ID
113705Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex, subunit 1|breast and ovarian cancer susceptibility protein 1
- Interactome
- Pathway
- Disease