Bcl2l1 (NM_001033671) Rat Tagged ORF Clone

CAT#: RR200468

  • TrueORF®

Bcl2l1 (Myc-DDK-tagged ORF) - Rat Bcl2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein, transcript variant 4, (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


Plasmid Map

Product Images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Bcl2l1
Synonyms bcl-X; Bcl-xl; Bcl2l; Bclx
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR200468 representing NM_001033671
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAGAGCAACCGGGAGCTGGTGGTTGACTTTCTCTCCTACAAGCTCTCCCAGAAAGGATACAGCT
GGAGTCAGTTTAGCGATGTCGAAGAGAACAGGACTGAAGCCCCAGAAGAAACTGAACCAGAAAGGGAGAC
CCCCAGTGCCATCAATGGCAACCCATCCTGGCACCTGGCGGATAGCCCCGCGGTGAATGGAGCCACTGGC
CACAGCAGCAGTTTGGATGCGCGGGAGGTAATCCCCATGGCAGCAGTGAAGCAAGCGCTGAGAGAGGCTG
GCGATGAGTTTGAACTGCGGTACCGGAGAGCATTCAGTGATCTAACATCCCAGCTTCATATAACCCCAGG
GACAGCATATCAGAGCTTTGAACAGGACACTTTTGTGGATCTCTACGGGAACAATGCAGCAGCCGAGAGC
CGGAAAGGCCAGGAGCGTTTCAACCGCTGGTTCCTGACGGGCATGACTGTGGCTGGTGTAGTTCTGCTGG
GCTCACTCTTCAGTCGGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR200468 representing NM_001033671
Red=Cloning site Green=Tags(s)

MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEPERETPSAINGNPSWHLADSPAVNGATG
HSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQDTFVDLYGNNAAAES
RKGQERFNRWFLTGMTVAGVVLLGSLFSRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001033671
ORF Size 510 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001033671.1, NP_001028843.1
RefSeq Size 2138 bp
RefSeq ORF 513 bp
Locus ID 24888
UniProt ID P53563
Cytogenetics 3q41
MW 19 kDa
Gene Summary inhibits neuronal apoptosis; may provide neuroprotection against ischemic brian injury [RGD, Feb 2006]

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