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> Anti-PROM1 antibody (31-80 N-Term)

Anti-PROM1 antibody (31-80 N-Term)

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Katalog-Nummer NB-22-6555-200ul

Size : 200µg

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Western blot analysis of lysate from A549 cells, using PROM1 Antibody.
  • Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of A549, SW620, HT29, Jurkat, mouse kidney, mouse lung cells using CD133 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

General Info

Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Prominin-1 (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: PROM1
Gene ID: 8842
Uniprot ID: PROM1_HUMAN
Immunogen Region: 41-90 aa
Specificity: CD133 Polyclonal Antibody detects endogenous levels of CD133 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the N-terminal region of human PROM1 at the amino acid range 41-90

Description

Post Translational Modifications Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Function May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Protein Name Prominin-1
Antigen Ac133
Prominin-Like Protein 1
Cd Antigen Cd133
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Microvillus Membrane
Cilium
Photoreceptor Outer Segment
Endoplasmic Reticulum
Endoplasmic Reticulum-Golgi Intermediate Compartment
Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid
Saliva
Seminal Fluid And Urine
Alternative Antibody Names Anti-Prominin-1 antibody
Anti-Antigen Ac133 antibody
Anti-Prominin-Like Protein 1 antibody
Anti-Cd Antigen Cd133 antibody
Anti-PROM1 antibody
Anti-PROML1 antibody
Anti-MSTP061 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance