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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-MOUSE IgG (H&L) (GOAT) Antibody Biotin Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins)

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Katalog-Nummer 610-106-121

Size : 1mg

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Rockland Immunochemicals

Contact local distributor :

Telefonnummer : +1 850 650 7790

Background

Anti-Mouse IgG Biotin Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Expand Background

Specifications for Mouse IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed

Product Details

Description: Goat Anti-Mouse IgG (H&L) Antibody Biotin Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-106-121

Synonyms: Goat Anti-Mouse IgG Secondary Antibody biotin Conjugation, Goat Anti-Mouse IgG Antibody biotin Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG biotin conjugated secondary antibody

Host Species: Goat

Specificity: IgG (H&L)

Conjugate: Biotin

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen: Mouse IgG whole molecule

Purity/Specificity: Mouse Secondary Antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Application Details

Tested Applications: ELISA

Suggested Applications: IHC - View References

Application Note: Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is ideal for western blot and are available in a variety of formats and conjugate types.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store second antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Rowlands JC et al. (2002). Altered mammary gland differentiation and progesterone receptor expression in rats fed soy and whey proteins. Toxicol Sci.

Applications

IHC, ICC, Histology

PubMed

Alam R et al. (1996). Increased MCP-1, RANTES, and MIP-1alpha in bronchoalveolar lavage fluid of allergic asthmatic patients. Am J Respir Crit Care Med.

Applications

EIA, ELISA

PubMed

Related Protocols

ELISA Protocol

Heat-induced Antigen Retrieval Protocol

Immunohistochemistry (IHC) Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.