Anti-HLA Class 1 Antigen A2 (FITC) Monoclonal Antibody

Katalog-Nummer H6098-07E-100ug

Size : 100ug

Marke : US Biological

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H6098-07E HLA Class 1 Antigen A2 (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P01892
Isotype
IgG2b
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells fused with spleen cells from Balb/c mice.

Recommended Negative Controls:
I1904-84S: IgG2b, Mouse (FITC)

Source:
Tissue culture supernatant

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 3H2205 (BB7.2)|Host: mouse|Source: human|Concentration: ~0.05mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC). |Purity: Purified by Protein G affinity chromatography.|Immunogen: Papain solubilized HLA-A2. The epitope appears to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.|Specificity: Recognizes the human HLA-A2 histocompatibility antigen.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Papain solubilized HLA-A2. The epitope appears to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the human HLA-A2 histocompatibility antigen.
References
1. Parham, P., Brodsky, F.M., Hum. Immunol. 3: 277-299 (1981). 2. Hogan, K.T., Brown, S.L., Hum Immunol. 33: 185-192 (1992). 3. Harig, S., et al., Blood 98: 2999-3005 (2001).