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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (APC) Monoclonal Antibody

Drucken

Katalog-Nummer C2262-36R-APC-100ul

Size : 100ul

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : US Biological

Weitere Informationen anfordern

Contact local distributor :

Telefonnummer : +1 850 650 7790

C2262-36R-APC CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (APC)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

P11215

Isotype

IgG1

Grade

Affinity Purified

Applications

IHC IP

Crossreactivity

Hu Mk

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD11b cell surface glycoprotein is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.||Applications:|Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry (Frozen): 1:100-1000|Optimal dilutions to be determined by the researcher.||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice. ||Positive Control Tissue:|Human Tosil||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: ICRF44|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rheumatoid synovial cells and human monocytes.|Specificity: Recognizes human CD11b. Species Crossreactivity: Cynomolgus monkey, baboon, Rhesus monkey. Does not react with feline. Reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Rheumatoid synovial cells and human monocytes.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes human CD11b. Species Crossreactivity: Cynomolgus monkey, baboon, Rhesus monkey. Does not react with feline. Reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release

References

1. Yoshino, M. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 2. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte. Leukocyte Typing IV. Oxford University Press 1058-1063. 3. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function. J. Cell Biol. 116: 1527-1535. 4. Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur. J. Immunol. 16: 1117-1123. 5. Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways. Blood 105: 341-9. 6. Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood 97: 2932-2940. 7. Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways. Blood 95: 3868-3877. 8. Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin. Haematologica. 96: 526-33. 9. Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes. J Am Coll Cardiol. 53: 962-71. 10. Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma. Am J Respir Crit Care Med. 164: 1403-9. 11. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow. J Pharmacol Exp Ther. 321: 656-62. 12. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells. Clin Exp Immunol. 130: 256-62. 13. Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis. Clin Appl Thromb Hemost. 17: 425-31.